Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for PP13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated PP13. A competitive inhibition reaction is launched between PP13 (Standards or samples) and HRP-conjugated PP13 with the pre-coated antibody specific for PP13. The more amount of PP13 in samples, the less antibody bound by HRP-conjugated PP13. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of PP13 in the sample. The color development is stopped and the intensity of the color is measured